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a549-hace2-tmprss2 cells (InvivoGen)

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InvivoGen a549-hace2-tmprss2 cells
A549 Hace2 Tmprss2 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 5842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a549+cells/A549-hACE2-TMPRSS2+Cells/custom%40a549-hace2tpsa%4010%2E64898%2F2026%2E08%2E14%2E744970
Average 96 stars, based on 5842 article reviews

a549-hace2-tmprss2 cells - by Bioz Stars, 2026-09

96/100 stars

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Article Title: RNA length and receptor usage define innate immune recognition across species
Article Snippet: Cells were transfected with an equimolar mixture of three pSpCas9(BB)-2A-Puro-hMDA5 or pSpCas9(BB)-2A-Puro-chMDA5 plasmids (2 μg total DNA per well) using Lipofectamine 3000 (Invitrogen, L3000001). .. In A549 cells, the culture medium was replaced 48 h post-transfection with fresh medium containing puromycin (1.5 μg/mL; InvivoGen ant-pr-1) for selection. ..

Stable Transfection:

Article Title: Linear ubiquitin chain assembly complex contributes to NLRP3-mediated pyroptotic cell death
Article Snippet: .. THP-1 and A549 cells were obtained from the American Type Culture Collection (ATCC), and THP-1 cells stably expressing HMGB1-Lucia were purchased from InvivoGen. ..

Expressing:

Article Title: TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions.
Article Snippet: After fixation with 10% neutral buffered formalin (20214, Muto Chemical, Tokyo, Japan), the cells were stained with 0.1% crystal violet (038-04862, Fujifilm Wako) in 20% ethanol. .. Human ACE2-expressing A549 cells were purchased from InvivoGen (San Diego, CA, USA) and transfected with the TM2-mCherry expression vector using FuGene 6. .. Transfected cells were selected and cultured with 500 μg/mL of Hygromycin B (Thermo Fisher Scientific).

Transfection:

Article Title: Single-cell heterogeneity in interferon induction potential is heritable and governed by variation in cell state
Article Snippet: .. A549 cells were transfected using Lipofectamine 3000 (Invitrogen) with the synthetic double-stranded RNA, pIC (Poly(I:C) LMW, InvivoGen), for the indicated time points. ..

Infection:

Article Title: Intrinsic OASL expression governs heterogeneity in interferon induction during influenza A virus infection.
Article Snippet: .. Supernatants from infected A549 cells were collected and incubated with HEK-Blue IFN-λ reporter cells (InvivoGen) in 96-well plates for 24 h. Following incubation, the supernatant from HEK-Blue cells was clarified by centrifugation at 2,000 rpm for 5 min at 4 °C. .. A volume of 20 to 50 μL was then incubated with QUANTI-Blue reagent (InvivoGen) according to the manufacturer’s protocol.

Incubation:

Centrifugation:

Virus:

Article Title: The hepatitis E virus capsid protein ORF2 counteracts cell-intrinsic antiviral responses to enable persistent replication in cell culture.
Article Snippet: .. Supernatants from A549 cells challenged with Mengo-Zn virus, Sendai virus (SeV), and poly(I:C) supernatant feeding for 24 h were collected and IFNβ secretion was measured using a bioluminescent human IFNβ ELISA (LumiKineTM Xpress hIFN-β 2.0, Invivogen) according to manufacturer’s instructions. .. 2 x 106 cells were pelleted, resuspended in 200 μL cytomix (120 mM KCl, 0.15 mM CaCl 2 , 10 mM KPO 4 , 25 mM HEPES, 2 mM EGTA, and 2 mM MgCl 2 ), and transferred to a 0.2 cm cuvette containing 500 ng high molecular weight (HMW) poly(I:C) (Invivogen) or control poly(C) (Sigma-Aldrich).

Enzyme-linked Immunosorbent Assay:

Cell Culture:

Article Title: A cell-based scrambling assay reveals the phospholipid headgroup preference of TMEM16F on the plasma membrane
Article Snippet: .. We cultured the HeLa and U2OS cells in DMEM media (Gibco, 10569044) supplemented with 10% FBS (Cultra Pure US origin, Axenia BioLogix), and A549 cells in advanced DMEM media (Gibco, 12491-015) supplemented with 2% fetal bovine serum (Gibco, 26140-079), 1 × GlutaMAX (Gibco, 35050-061), and 100 μg/mL Normocin (InvivoGen, ant-nr-05). .. Cells were dislodged for passaging with TrypLE (Gibco, A1217701) after washing twice with ambient temperature DPBS (Gibco, 14190136).

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$Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.$

Journal: Advances in Radiation Oncology

Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

doi: 10.1016/j.adro.2026.102051

Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated